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MPNS COST Action 1205
Advances in Optofluidics: Integration of Optical Control and Photonics with Microfluidics

European Cooperation in Science and Technology
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DTU Nanotech

DTU Nanotech, Denmark

Project Title:

Optically actuated micro and nanofluidics for DNA analysis


Nanofluidic devices, fabricated in plastic by injection moulding or direct nanoimprint are used as a platform for analysis of single DNA molecules in solution. In nanoconfinement, DNA molecules will unwind and stretch such that their inherent information is more readily accessible. For the experiments, the DNA molecules are stained and visualized by fluorescence microscopy. We use these techniques to develop a next generation of DNA sequencers based on single molecules. In this path, we study devices and protocols to manipulate and sort single DNA molecules. Aiming for cheap and single use devices, which can be produced by production grade methods, optical actuation alone is used for the devices. We investigate the potential of polymer photonic crystals and nanoplasmonic resonators for sensing. Optothermal actuation of the nanofluidic devices is used for moving, exciting or trapping individual molecules, or even their parts.

Funding sources:

EC FP7 HEALTH: CellOMatic (2012-15)
EC FP7 NMP: Plast4future (2013-16)
Danish Council for Strategic Reserch: PolyNano (2012-2016)

WG1: Integrated Microfluidic Photonics

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